Nuclear Magnetic Resonance Facility
The University of Cincinnati Nuclear Magnetic Resonance (NMR) Facility is located in Crosley Tower in rooms 123 and 108. This facility supports the research of faculty and students in the Department of Chemistry, as well as other departments, universities, and industry. The NMR facility offers both solution and solid-state capabilities with its three NMR spectrometers ranging from 9.4 to 11.7 T. Contact Dr. Alex Greenwood (greenwa2@ucmail.uc.edu, 513-556-9211) for instrument training, experiment scheduling, or more information.
Instrumentation
Bruker NEO 400
Bruker NEO 400 (123 Crosley Tower)
Magnet: Bruker Ascend 9.4 T, 54 mm (narrow) bore, BOSS-3 36 shim system
Console: Bruker NEO 400, two rf channels, BLABBH500/100 2-channel amplifier system, HPPR II preamplifier unit
Probes:
Bruker 4mm solid-state MAS HX iProbe, 109Ag-19F observe with 1H decoupling and observe, up to 15 kHz spinning, automatic tuning and matching accessory, VT range from -80 to 200 C (PI MAS-400-S1-4.0mm BB/F-H)
Bruker 5 mm SMART 109Ag-31P and 19F observe with 1H decoupling and observe, Z-axis PFG, automatic tuning and matching accessory, VT range from 150 to +150 °C (PI HR-400-S1-BBF/H/D-5.0-Z SP)
Bruker 5 mm Quattro Nucleus (QNP) 31P/13C/15N and 1H, Z-axis PFG (PH QNP 400S1 P/C/N-H-D-05 Z)
Computer: HP z440 running TopSpin 4.3.0 in CentOS 7.5.
Accessories: SampleXpress Lite autosampler (16 sample positions), BCU I VT control unit
Bruker AV 400
Bruker AV 400 (108 Crosley Tower)
Magnet: Bruker Ultrashield 9.4 T, 54 mm (narrow) bore, BOSS-1 20 shim system
Console: Bruker Avance I 400, two rf channels, BLA2BB150/60 2-channel amplifier system, HPPR preamplifier unit
Probes:
Bruker 5 mm 109Ag-31P and 19F observe with 1H decoupling and observe, Z-axis PFG, automatic tuning and matching accessory, VT range from -150 to +150 °C (PA BBO 400S1 BBF-H-D-05 Z)
Bruker 5 mm Inverse 109Ag-31P decoupling and observe with 1H decoupling and observe, Z-axis PFG, VT range from -150 to +150 °C (PH BBI 400S1 H-B-D-05 Z)
Computer: HP z420 running ICON-NMR/Topspin 2.1PL6 in CentOS 5.10
Accessories: SampleXpress Lite autosampler (16 sample positions), BCU 05 VT control unit
AV 500
Bruker AV 500 (123 Crosley Tower)
Magnet: Bruker Spectrospin 11.7 T, 54 mm (narrow) bore, BOSS-3 36 shim system
Console: Bruker AVIII 500, three rf channels, BLAXH2H300/100/150 + BLAX300 3-channel amplifier system, HPPR/2 preamplifier unit
Probes: Bruker 5 mm 109Ag-31P observe with 1H decoupling and observe, Z-axis PFG, automatic tuning and matching accessory, VT capabilities (BBO 500S2 BB-H-D-05 Z)
Bruker 5 mm Inverse 109Ag-31P decoupling and observe with 1H decoupling and observe, Z-axis PFG (PH BBI 500S2 H-BB-Z-05 Z)
Bruker 5 mM Inverse Triple resonance (TXI) 1H-13C/15N, Z-axis PFG
Computer: HP z400 running ICON-NMR/Topspin 3.5PL7 in CentOS 5.11
Accessories: SampleXpress Lite autosampler (16 sample positions), BCU 05 VT control unit
People
Alex Greenwood, Manager
(greenwa2@ucmail.uc.edu, 513-556-9211)
Dr. Alex Greenwood serves as the manager of the UC NMR facility. Alex has a broad background in NMR spectroscopy, with experience applying multidimensional NMR to proteins, peptides, and small molecules in solution and in the solid state both using magic-angle spinning (MAS) and oriented-sample (OS) approaches. Generally, his focus has been on computational approaches for informing on the structures and binding properties of biomolecules. As a PhD student he applied NMR lineshape analysis to determine the catalytic and binding rates of isomerase enzymes. His more recent academic contributions include applying density functional theory (DFT)-based chemical shift calculations to solid-state NMR-based structure calculations of both proteins and pharmaceuticals.
Rates
| Instrument | 1-8 hr |
8-24 hr |
24+ hr |
|---|---|---|---|
| NEO 400 | $6.00 | $5.00 | $3.00 |
| AV 400 | $6.00 | $5.00 | $3.00 |
| AV 500 | $6.00 | $5.00 | $3.00 |
| Instrument |
Academic | Industry |
|---|---|---|
| NEO 400 | $10.00 | $50.00 |
| AV 400 | $10.00 | $50.00 |
| AV 500 | $10.00 | $50.00 |